| 中文关键词: HL-60细胞；维生素D受体 1，25二羟基维生素D3 |
| 英文关键词:HL-60 cell vitamin D receptor 1,25(OH)2D3 |
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| 摘要点击次数: 1975 |
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| 中文摘要: |
| 摘 要:［目的］ 研究1，25二羟基维生素D3［1，25(OH)2D3］对人HL-60细胞株生长、分化、凋亡及对维生素D受体(VDR)蛋白表达的影响。［方法］ 在体外用不同浓度的1，25(OH)2D3作用于对数生长期的HL-60细胞。甲基噻唑基四唑(MTT)比色法分析细胞增殖抑制作用，硝基四氮唑蓝(NBT)还原实验法分析HL-60细胞的分化指标，末端脱氧核糖核酸转移酶介导的dUTP缺口末端标记染色法(TUNEL)检测细胞晚期凋亡的变化，免疫细胞化学法检测VDR 蛋白表达。［结果］ 不同浓度的1，25(OH)2D3作用后均可抑制HL-60细胞的生长，呈剂量和时间依赖性；可诱导HL-60细胞向成熟粒细胞分化并促其凋亡。1，25(OH)2D3作用前后HL-60细胞均表达VDR蛋白，药物作用后VDR蛋白表达上调。［结论］ 1，25(OH)2D3在一定浓度范围内可抑制HL-60细胞增殖，诱导细胞分化及凋亡，使VDR蛋白表达上调。 |
| 英文摘要: |
| Abstract: ［Purpose］ To investigate the effects of 1，25-Dihydroxyvitamin D3 ［1，25(OH)2D3 ］ at different concentrations on inhibition，differentiation，apoptosis and the expression of vitamin D receptor (VDR) protein in human leukemia cell line HL-60. ［Methods］ HL-60 cell line was treated by 1，25(OH)2D3 with different concentrations. Methy thiazoly tetrazolium (MTT) assay was used to evaluate the cell proliferation. The differentiation of HL-60 cells was detected by nitro blue tetrazolium (NBT) reduction test. The cells late apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling staining assay(TUNEL). The expression of VDR protein was detected by immunocytochemistry technique. ［Results］ 1，25(OH)2D3 with different concentrations could significantly inhibit the proliferation of HL-60 cells in a dose-dependent and time-dependent manner. 1，25(OH)2D3 could remarkably induce HL-60 cells to differentiate towards mature granulocyte and enhance cell apoptosis rate. VDR protein expressed in the all HL-60 cell line before and after the action of 1，25(OH)2D3. VDR protein expression up-regulated after drug action. ［Conclusions］ 1，25(OH)2D3 at a certain concentration range can significantly inhibit the proliferation and induce differentiation and apoptosis in HL-60 cell line. 1，25(OH)2D3 can increase expression of VDR protein in HL-60 cell line. |
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