| 张苗尊,章 维.错配修复基因hMSH2和hMLH1在结直肠癌组织中的表达及意义[J].中国肿瘤,2013,22(4):308-311. |
| 错配修复基因hMSH2和hMLH1在结直肠癌组织中的表达及意义 |
| Expression of Mismatch Repair Gene hMLH1 and hMSH2 in Colorectal Carcinoma and Its Significance |
| 投稿时间：2012-11-17 |
| DOI：10.11735/j.issn.1004-0242.2013.04.A201303078 |
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| 中文关键词: 肠肿瘤 错配修复基因 hMLH1 hMSH2 免疫组化 |
| 英文关键词:colorectal carcinoma mismatch repair genes hMLH1 hMSH2 immunohistochemical method |
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| 中文摘要: |
| 摘 要：［目的］ 评价错配修复基因hMLH1 和hMSH2在结直肠癌中的表达及意义。［方法］ 经病理学确诊的结直肠癌手术切除标本85例，术前均未接受过放疗或化疗，免疫组化检测hMLH1 和hMSH2 蛋白表达情况。［结果］ 85例结直肠癌组中，hMSH2 缺失率为69.4%(59/85)，hMLH1蛋白缺失率为78.8%(67/85)。hMSH2蛋白缺失率在T1、T2、T3和T4期结直肠肿瘤中的缺失率分别为50.0%、43.8%、71.1%和82.8%，随T分期增加而增加（χ2=11.037，P=0.012）。有淋巴结转移者的hMSH2蛋白缺失率达88.57%（31/35），而无淋巴结转移患者的hMSH2蛋白缺失率为56.0%（28/50）（χ2=10.287，P=0.001）。hMLH1蛋白缺失率与肿瘤分化程度和T分期相关，但与性别、年龄、肿瘤部位、N分期和M分期均无关。［结论］ 在结直肠癌组织中存在hMSH2 和hMLH1 蛋白缺失，且hMSH2 和hMLH1 蛋白缺失与肿瘤T分期和分化程度相关。检测hMLH1 和hMSH2 蛋白表达对预后判断有一定的参考价值。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the expression of mismatch repair gene human mutl homolog 1(hMLH1) and human mutl homolog 2(hMSH2) in colorectal carcinoma and its significance. ［Methods］ A total of 85 pathologically proved patients with colorectal carcinoma undergoing surgical resection were included in the study. All the patients did not undergo preoperative chemotherapy or radiotherapy. The expression of hMLH1 and hMSH2 protein was detected by immunohistochemical method.［Results］ The loss rate of hMSH2 and hMLH1 expression in cancer tissue was 69.4%(59/85)，78.8%(67/85) respectively. The loss rate of hMSH2 expression in patients with T1，T2，T3，T4 was 50.0%，43.8%，71.1% and 82.8% respectively. The loss rate of hMSH2 expression increased with T stage increasing（χ2=11.037，P=0.012). The loss rate of hMSH2 expression in patients with lymph node metastases was 88.57%（31/35），but 56.0%（28/50）in patients without lymph node metastases with significant difference（χ2=10.287，P=0.001). The loss rate of hMLH1 expression was correlated to tumor differentiation and T stage，but not correlated to gender，age，tumor site，N stage and M stage. ［Conclusion］ Loss expression of hMLH-1 and hMSH-2 protein exists in colorectal carcinoma. Expression loss of hMLH-1 and hMSH-2 protein correlates to tumor differentiation and T stage. Combined detection of hMLH-1 and hMSH-2 expression might be useful for evaluating prognosis of colorectal cancer. |
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