| 罗顺祥,戈 伟,刘梓良.金水鲜胶囊联合放射治疗对肺腺癌A549细胞HIF-1α和 EGFR mRNA表达的影响[J].中国肿瘤,2013,22(10):830-833. |
| 金水鲜胶囊联合放射治疗对肺腺癌A549细胞HIF-1α和 EGFR mRNA表达的影响 |
| Effect of Jinshuixian Capsule Combined with Radiotherapy on Expression of HIF-1α and EGFR mRNA in A549 Cell of Lung Adenocarcinoma |
| 投稿时间：2013-01-02 |
| DOI：10.11735/j.issn.1004-0242.2013.10.A014 |
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| 中文关键词: 金水鲜胶囊 放射治疗 A549细胞 HIF-1α EGFR |
| 英文关键词:inshuixian capsules radiotherapy lung adenocarcinoma A549 HIF-1 α EGFR |
| 基金项目:中国癌症基金会资助项目(2009cfcxjs102) |
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| 中文摘要: |
| 摘 要：［目的］ 研究金水鲜胶囊联合放疗对肺腺癌A549细胞增殖的抑制作用以及HIF-1α和 EGFR mRNA表达的影响。［方法］将处于对数期生长的A549细胞随机分为4组（空白对照组、放射治疗组、金水鲜胶囊组和放疗治疗+金水鲜胶囊组），MTT法计算细胞生长抑制率，RT-PCR技术检测HIF-1α和EGFR mRNA的含量变化。［结果］各组肺腺癌A549细胞生长受到不同程度的抑制，以金水鲜胶囊联合放疗组最明显，差异有统计学意义（P<0.05）。除放疗组外，各组HIF-1α和EGFR mRNA表达均下降。48h时，金水鲜胶囊组及金水鲜胶囊联合放疗组与空白对照组、放疗组相比，HIF-1α和EGFR mRNA表达差异有统计学意义（P<0.05）。［结论］ 金水鲜联合放疗可抑制肺腺癌细胞增殖，下调HIF-1α和EGFR mRNA的表达。 |
| 英文摘要: |
| Abstract:［Purpose］ To investigate the inhibitory effect and the expression of HIF-1α mRNA and EGFR mRNA of Jinshuixian capsule combined with radiotherapy in lung adenocarcinoma cell line A549.［Methods］ A549 cells in log phase were randomly divided into 4 groups:control group，radiotherapy group，Jinshuixian group and Jinshuixian plus radiotherapy group . The cell growth inhibition rate was evaluated by MTT method，and HIF-1 α and EGFR mRNA were detected by RT-PCR technique.［Results］ The cell growth in lung adenocarcinoma cell line A549 was inhibited with the significant inhibition in Jinshuixian plus radiotherapy group(P<0.05). In addition to radiotherapy group，expression of HIF-1 α and EGFR in other group was decreased. The level of HIF-1α and EGFR mRNA was significant different in Jinshuixian group，Jinshuixian combined radiotherapy group compared to control group and radiotherapy group(P all<0.05). ［Conclusion］ Jinshuixian combined with radiotherapy could inhibit the proliferation of lung adenocarcinoma cells，and decrease the expression of HIF-1 α and EGFR mRNA. |
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