| 陈晓霞,李 剑.miR-34a调控肺癌EGFR耐药细胞株中MET信号通路探讨[J].中国肿瘤,2013,22(12):1020-1024. |
| miR-34a调控肺癌EGFR耐药细胞株中MET信号通路探讨 |
| miR-34a Regulating MET Signaling Pathway in EGFR Resistant Lung Cancer Cell Lines |
| 投稿时间：2013-06-17 |
| DOI：10.11735/j.issn.1004-0242.2013.12.A014 |
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| 中文关键词: 肺癌 获得性耐药 miR-34a MET |
| 英文关键词:lung cancer acquired resistance miR-34a MET |
| 基金项目:浦东新区卫生局卫生科技项目（PW2011A-18） |
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| 中文摘要: |
| 摘 要：［目地］ 研究肺癌耐药细胞株HCC827/GR中MET信号通路的调控，明确肺癌获得性耐药的分子机制。［方法］ 使用HCC827细胞［epidermal growth factor receptor（EGFR）基因19外显子缺少的肺腺癌细胞株］，在此细胞的基础上培养吉非替尼耐药细胞株。检测耐药细胞株中MET的表达，并使用RT-PCR的方法检测miR-34a的表达；使用TargetScan等生物信息学软件预测miR-34a的下游靶点，并在细胞内验证miR-34a是否可以调控MET的表达。［结果］ 与HCC827细胞相比，HCC827/GR细胞株对吉非替尼明显耐药。在耐药HCC827/GR细胞株中，miR-34a低表达，MET高表达，而在HCC827细胞株中，miR-34a高表达，MET低表达。TargetScan生物信息学软件提示，MET是miR-34a的下游靶点之一。将携带荧光报告基团和MET 3′UTR的载体与miR-34a共转染入细胞后荧光度下降。［结论］ miR-34a可能通过调控靶基因MET而参与EGFR-TKI的获得性耐药。 |
| 英文摘要: |
| Abstract：［Purpose］ To study the miR-34a regulating MET signaling pathway in EGFR resistant lung cancer cell lines，and to investigate the mechanisms of acquired resistance of lung cancer.［Methods］ On the basis of HCC827 cells (epidermal growth factor receptor gene exon 19 deletions in lung adenocarcinoma cell lines)，the gefitinib resistant cell lines were cultured. the expression of MET and miR-34a in the drug-resistant cell lines were detected by RT-PCR method. The downstream target of miR-34a were predicted by bioinformatics software such as TargetScan. Whether miR-34a could regulate the expression of MET in the intracellular was verified. ［Results］ HCC827/GR cell lines resistant to gefitinib approximately 100-fold compared with HCC827 cells. Low expression of miR-34a and high expression of MET in the resistant cell lines was found，while high expression of miR-34a and low expression of MET in the HCC827 cells. TargetScan bioinformatics software indicated MET is one of downstream target of miR-34a. The fluorescence was decreased when the vectors carried fluorescent reporter group and MET 3′UTR and miR-34 were co-transfected into cells.［Conclusion］ MiR-34a involves in EGFR-TKI acquired resistance.It might regulate target genes MET. |
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