| 余新民,张爱琴,陈莎莎.软癥散结合剂对食管癌移植瘤BAT-26及D3S1067微卫星不稳定性的影响[J].中国肿瘤,2014,23(3):234-238. |
| 软癥散结合剂对食管癌移植瘤BAT-26及D3S1067微卫星不稳定性的影响 |
| Impact of RuanZhengSanMixture on Microsatellite Instability in BAT-26 and 3S1067 of Esophageal Cancer Xenografts |
| 投稿时间：2013-10-24 |
| DOI：10.11735/j.issn.1004-0242.2014.03.A013 |
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| 中文关键词: 软癥散结合剂 人食管癌移植瘤 微卫星不稳定性 BAT-26 D3S1067 |
| 英文关键词:RuanzhengsanMixture esophageal cancer xenografts microsatellite instability BAT-26 |
| 基金项目:浙江省中医药科技计划项目(20102B008) |
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| 中文摘要: |
| 摘 要：［目的］ 观察软癥散结合剂对裸鼠人食管癌移植瘤的抑制作用，及其对瘤体BAT-26 及D3S1067 微卫星不稳定性（MSI）的影响。［方法］ 建立裸鼠皮下食管癌移植瘤模型，模型建成后随机分为5组：正常组、生理盐水组、软癥散结合剂低、中、高剂量组。实验15d后剥取肿瘤并称重，计算抑瘤率。荧光PCR法检测移植瘤组织中BAT-26 及D3S1067MSI的表达。［结果］低、中、高剂量软癥散结合剂对裸鼠的移植瘤抑制率分别为27.11%、47.69%和35.56%。软癥散结合剂中剂量组D3S1067 MSI发生率显著性低于生理盐水组（P＜0.05）。各组BAT-26位点MSI表达均很低，且无统计学差异。［结论］ 软癥散结合剂能抑制人食管癌细胞移植瘤生长，这一作用可能与其下调肿瘤细胞D3S1067位点的微卫星不稳定性相关。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate RuanZhengSanMixture inhibitory effect on esophageal cancer transplanted tumor in nude mouse，and the effect on microsatellite instability(MSI) in BAT-26 and 3S1067.［Methods］ Nude mice subcutaneously transplanted esophageal tumor model was established，then randomly divide into 5 groups：normal group，saline group，Low，medium and high dose RuanZhengSanMixture group. Transplantation tumor was weighed 15 days after experiment，then inhibition rate was calculated. MSI in BAT-26 and 3S1067 was detected by Real-time PCR.［Results］ The inhibition rate in low，medium and high dose RuanZhengSanMixture group was 27.11%、47.69% and 35.56% respectively. The MSI rate of D3S1067 in middle dose group was less than that in saline group(P<0.05). MSI in the BAT-26 in all the five groups was low with significant differences.［Conclusion］ RuanZhengSanMixture can inhibit the growth of human esophageal cancer cell xenografts.This effect might be associated with down regulation of microsatellite instability in D3S1067 locus. |
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