| 宋启斌,褚玉新,胡伟国.GW843682X对鼻咽癌细胞周期和凋亡的影响[J].中国肿瘤,2014,23(10):860-864. |
| GW843682X对鼻咽癌细胞周期和凋亡的影响 |
| The Influence of GW843682X on Cell Cycle and Apoptosis of Nasopharyngeal Carcinoma Cell Line |
| 投稿时间：2014-01-27 |
| DOI：10.11735/j.issn.1004-0242.2014.10.A015 |
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| 中文关键词: GW843682X 鼻咽癌 细胞周期 细胞凋亡 |
| 英文关键词:GW843682X nasopharyngeal carcinoma cell cycle cell apoptosis |
| 基金项目:国家自然科学基金(81372407) |
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| 摘要点击次数: 2264 |
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| 中文摘要: |
| 摘 要：［目的］ 研究GW843682X对鼻咽癌5-8F细胞周期和凋亡的影响。［方法］ 培养鼻咽癌5-8F细胞，0、6.25、12.5、25、50、100、200和400nmol/L的GW843682X处理后，用CCK8试剂检测药物对细胞增殖的影响。分别以0、250，500，1000nmol/L的GW843682X处理5-8F细胞48h后，用PI单染流式细胞仪检测药物对5-8F细胞周期的影响。用Annexin V-FITC双染细胞后，经流式细胞仪检测细胞凋亡的变化。［结果］ CCK8比色结果显示，GW843682X能抑制鼻咽癌5-8F细胞的增殖，当药物浓度大于50nmol/L时，细胞增殖显著受抑。细胞周期检测结果显示，与阴性对照组比较，GW843682X增加了G2/M期细胞阻滞，减少了G0/G1期细胞比例(P<0.05)。细胞凋亡检测结果显示，与对照组相比，随着GW843682X浓度的增加，凋亡细胞所占比例逐渐增大，当药物浓度达到500nmol/L和1000nmol/L时，凋亡细胞所占比例显著增加(P<0.05)。［结论］ GW843682X能够显著抑制鼻咽癌5-8F细胞的增殖，可以诱导细胞阻滞于G2/M期，其诱导5-8F细胞凋亡呈一定的剂量依赖性。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the influence of GW843682X on the cell cycle and apoptosis of nasopharyngeal carcinoma cell line 5-8F. ［Methods］ 5-8F cells were cultured and treated with 0，6.25，12.5，25，50，100，200，400nmol/L GW843682X. The influence of GW843682X on cell proliferation was detected by CCK8 assay. 5-8F cells treated with GW843682X (0，250，500，1000nmol/L) for 48h. The cell cycle were detected by flow cytometry with PI simple staining. Cell apoptosis were tested by Annexin V-FITC double staining by flow cytometry. ［Results］ The result of CCK8 assay indicated that GW843682X inhibited the proliferation of 5-8F cells． When the concentration of GW843682X was more than 50nmol/L，cell proliferation was remarkably inhibited. Cell cycle detection revealed that，compared to control group，GW843682X increased G2/M mitosis arrest，while decreased G0/G1 cells(P<0.05). Cell apoptosis assay also revealed that increased concentration of GW843682X induced higher proportion of apoptotic cells. When the concentration of GW843682X reached 500nmol/L and 1000nmol/L，the proportion of apoptotic cells increased markedly(P<0.05).［Conclusion］ GW843682X can remarkably inhibit the proliferation of 5-8F cells，induce mitosis arrest at G2/M phase，and induce cell apoptosis in a dose-dependent manner. |
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