| 邓守明,石 芳.EGCG对体外培养喉癌Hep-2细胞生物学行为的影响[J].中国肿瘤,2015,24(1):64-67. |
| EGCG对体外培养喉癌Hep-2细胞生物学行为的影响 |
| Effect of EGCG on Biological Behaviour of Human Laryngeal Cancer Hep-2 Cells in vitro |
| 投稿时间：2014-07-22 |
| DOI：10.11735/j.issn.1004-0242.2015.01.A014 |
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| 中文关键词: 表没食子儿茶素没食子酸酯 喉癌 血管内皮生长因子 |
| 英文关键词:epigallocatechin-3-gallate laryngeal cancer vascular endothelial growth factor |
| 基金项目:湖北省自然基金项目（2008CDZ053） |
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| 摘要点击次数: 1874 |
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| 中文摘要: |
| 摘 要：［目的］ 研究表没食子儿茶素没食子酸酯（EGCG）对体外培养喉癌Hep-2细胞增殖、凋亡和侵袭的影响。［方法］ 采用CCK-8法检测EGCG对喉癌细胞增殖的影响；流式细胞法检测细胞凋亡及细胞周期；细胞划痕法和Transwell法检测体外侵袭；蛋白质免疫印迹法检测血管内皮生长因子（VEGF）表达水平。 ［结果］ EGCG 5~160mg/L作用Hep-2细胞24~72h，可对细胞增殖产生明显的抑制作用，其24、48、72h的IC50值分别为139.6、38.7、24.3mg/L。10、20、40mg/L EGCG作用48h，Hep-2细胞凋亡率分别为（3.1±0.2）%、（9.9±0.6）%、（11.7±1.2）%，细胞被阻滞于G1期。5、10、20、40 mg/L EGCG作用48h，Hep-2细胞侵袭抑制率分别为（19.6±2.2）%、（37.2±3.8）%、（49.3±4.1）%和（62.6±3.4）%，VEGF蛋白表达下调率分别为（15.8±2.2）%、（51.4±2.1）%、（78.4±3.1）%和（89.6±2.4）%，与对照组相比差异均有统计学意义。［结论］ EGCG可通过下调VEGF蛋白表达对喉癌细胞产生抑制增殖、诱导凋亡和降低侵袭等作用。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the effects of epigallocatechin-3-gallate(EGCG) on biological behaviour of human laryngeal cancer Hep-2 in vitro. ［Methods］ The growth and proliferation of Hep-2 cell were detected by CCK-8 assay . Cell apoptosis and cell cycle were detected by the flow cytometry. Cell invasive ability was measured with cell scratch assay and Transwell method. The expression of vascular endothelial growth factor(VEGF) was detected by Western blot. ［Results］ After treatment with EGCG 5~160mg/L for 24~72h，the proliferation of Hep-2 cell could be inhibited obviously. The IC50 value in 24、48、72h was 139.6，38.7，24.3mg/L respectively； After treatment with EGCG 10、20、40 mg/L for 48h，The apoptosis rate of Hep-2 cell was (3.1±0.2)%、(9.9±0.6)%、(11.7±1.2)% respectively； and the cell phase was arrested in G1 period. After treatment with 5、10、20、40 mg/L EGCG for 48h，the invasive inhibitory rate to Hep-2 cells was (19.6±2.2)%、(37.2±3.8)%、(49.3±4.1)% and(62.6±3.4)% respectively. The down regulating rate of VEGF protein was (15.8±2.2)%、(51.4±2.1)%、(78.4±3.1)% and (89.6±2.4)% respectively. ［Conclusion］ EGCG suppresses the proliferation，induces apoptosis and decreases invasion in Hep-2 cells by down-regulating the expression of VEGF. |
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