| 杨 栋,张培彤,王耀焓.川芎嗪对PG干细胞样细胞VEGF、HIF-1α蛋白表达的影响[J].中国肿瘤,2015,24(3):234-240. |
| 川芎嗪对PG干细胞样细胞VEGF、HIF-1α蛋白表达的影响 |
| Effect of TMP on the Expression of VEGF and HIF-1α of PG CSC Like Cells |
| 投稿时间：2014-09-16 |
| DOI：10.11735/j.issn.1004-0242.2015.03.A014 |
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| 中文关键词: 干细胞 川芎嗪 血管生成 VEGF HIF?鄄1α |
| 英文关键词:CSC cell TMP angiogenesis VEGF HIF-1α |
| 基金项目:国家自然科学基金资助项目（81173450） |
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| 中文摘要: |
| 摘 要：［目的］ 研究活血药川芎有效成分川芎嗪对高转移性人巨细胞肺癌细胞PG-BE1干细胞样细胞VEGF、HIF-1α蛋白表达的影响。［方法］ 利用无血清成球培养法分选PG-BE1中的干细胞亚群并验证其干性。用含不同浓度川芎嗪的培养液干预PG-BE1干细胞样细胞，同时设立空白对照组及顺铂（DDP）组，采用ELISA法检测PG-BE1亲代细胞、干细胞样细胞（常氧环境和低氧环境）以及不同浓度药物干预后的PG-BE1干细胞样细胞的VEGF表达，采用Wester blot法检测各组HIF-1α蛋白表达。［结果］ PG-BE1干细胞样细胞VEGF表达强于其亲代细胞（P<0.001），VEGF在低氧环境中的表达高于常氧环境（P<0.001），不同浓度川芎嗪均可下调VEGF表达（P＜0.001），且具有剂量依赖性；PG-BE1干细胞样细胞HIF-1α表达强于其亲代细胞（P＜0.001），HIF-1α在低氧环境中的表达高于常氧环境（P=0.001），不同浓度川芎嗪均可下调HIF-1α表达（P＜0.001），但不同剂量之间差异无统计学意义（P=0.477)。［结论］ PG-BE1干细胞样细胞VEGF、HIF-1α表达高于其亲代细胞，低氧环境下VEGF、HIF-1α表达上调，川芎嗪对VEGF、HIF-1α表达有一定的抑制作用。 |
| 英文摘要: |
| Abstract：［Purpose］ To explore the effect of TMP（the effective composition of Chuanxiong） on the expression of VEGF and HIF-1α of high metastatic human lung cancer cells(PG-BE1). ［Methods］ Firstly，we sorted the CSC subgroups from the PG-BE1 CSC cells the serum free sphere culture method and then verified its stem cell propertiest.Based on the above experiment，we intervened the PG-BE1 CSC cells with different concentrations of TMP.Then we detected the effect of expression of VEGF of PG-BE1 CSC cells intervened by the different concentrations of TMP and the difference of the expression of VEGF between the PG-BE1 CSC like cells and parental cells by ELISA method. Finally，we detected the expression of HIF-1α of PG-BE1 CSC cells intervened by the different concentrations of TMP and the difference of the expression of HIF-1α between the PG-BE1 CSC like cells and parental cells by Western blot method.［Results］ PG-BE1 CSC cells expressed higher VEGF compared with parental cells(P<0.001).PG-BE1 CSC like cells expressed higher VEGF in hypoxia environment(P<0.001). Different concentrations of TMP can inhibit the expression of VEGF(P<0.001) and there were dose dependence between different concentrations. PG-BE1 CSC cells expressed higher HIF-1α compared with parental cells(P<0.001). PG-BE1 CSC like cells expressed higher VEGF in hypoxia environment(P=0.002). Different concentrations of TMP can inhibit the expression of HIF-1α(P<0.001)，but there were no dose dependence between different concentrations(P=0.477).［Conclusion］ PG-BE1 CSC like cells expressed high VEGF and HIF-1α. PG-BE1 CSC like cells expressed higher VEGF and HIF-1α in hypoxia environment .TMP can inhibit the expression of VEGF and HIF-1 α to a certain extent. |
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