| 方 陈,周 曦,王永生.MiR-214对人肺腺癌细胞PC9增殖及凋亡的研究[J].中国肿瘤,2015,24(10):860-863. |
| MiR-214对人肺腺癌细胞PC9增殖及凋亡的研究 |
| The Effect of MiR-214 on the Growth and Apoptosis of Human Lung Adenocarcinoma Cell Line PC9 |
| 投稿时间：2015-05-03 |
| DOI：10.11735/j.issn.1004-0242.2015.10.A013 |
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| 中文关键词: 肺肿瘤 miR-214 增殖 凋亡 |
| 英文关键词:lung cancer miR-214 growth apoptosis |
| 基金项目:国家自然科学基金青年基金（81301882）；中央高校基金（20620140118） |
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| 中文摘要: |
| 摘 要：［目的］ 探讨miR-214对肺腺癌细胞PC9增殖和凋亡的影响。［方法］ 培养人肺腺癌细胞株PC9（EGFR基因19外显子缺失，对EGFR-TKI吉非替尼敏感），将miR-214类似物(miR-214 mimic)及对照物（scramble）转染至PC9细胞中，在不同的时间点（24、48、72h）使用MTT检测细胞的增殖；转染细胞后，加入吉非替尼共培养48h后，使用流式细胞仪检测细胞凋亡；同时建立PC9荷瘤小鼠，观察荷瘤小鼠瘤体的生长曲线，及尾静脉注射miR-214抑制剂（antigomiR-214）后观察荷瘤小鼠瘤体的生长。［结果］ 在PC9细胞中，miR-214 mimic被转染入细胞后，48h及72h后细胞增殖率分别增加了27.9%和18.7%，相比转让了对照组的细胞差异均有统计学意义（P均<0.05）。吉非替尼可以诱导PC9细胞凋亡，但转染miR-214mimic后细胞凋亡率由86.5%下降为28.5%。在荷瘤小鼠中抑制miR-214后，小鼠瘤体明显缩小（P<0.05）。［结论］ miR-214可促进肺腺癌细胞增殖及抗凋亡，可能可作为肺腺癌治疗的新靶点。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the effect of miR-214 on the growth and apoptosis of PC9 in vitro and in vivo. ［Methods］ PC9 cells (EGFR exon 19del and sensitive to EGFR-TKI，such as gefitinib) were cultured and miR-214 mimic or scramble were transfected into PC9 cells；the growth was detected by MTT at different time points(24，48 and 72h). Next，PC9 cells were induced apoptosis by gefitinib after 48h of miR-214 or scramble transfection，and the apoptosis rate was analyzed by Annexin-FITC. The xenograft model of PC9 mice was established and miR-214 inhibitor were transfered into the mice，the xenograft tumor volume and growth curve were observed. ［Results］ Compared with the control group，the PC9 cells growth was increased 27.9% and 18.7% when miR-214 mimic tranfected into PC9 cells after 48 or 72h(all P＜0.05). Gefitinib could induce the apoptosis of PC9 cells，but with the transfection of miR-214 mimic，the apoptosis rate decreased from 86.5% to 28.5%. When miR-214 was inhibited by antagomir-214 in vivo，xenograft tumor volume decreased significantly(P＜0.05). ［Conclusion］ MiR-214 can promote the growth and reduce apoptosis of PC9 cells；it might be a potential therapeutic target lung adenocarcinoma in future. |
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