| 张克勤,赵文飞,陆月香.热化疗对ARH-77细胞株增殖抑制、凋亡及Bcl-2表达影响的研究[J].中国肿瘤,2016,25(2):138-142. |
| 热化疗对ARH-77细胞株增殖抑制、凋亡及Bcl-2表达影响的研究 |
| The Influence of Thermochemotherapy on Inhibition of Proliferation，Apoptosis and Bcl-2 Expression in ARH-77 Cell Lines |
| 投稿时间：2015-07-24 |
| DOI：10.11735/j.issn.1004-0242.2016.02.A012 |
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| 中文关键词: 热化疗 ARH-77细胞株 凋亡 Bcl-2 |
| 英文关键词:thermochemotherapy ARH-77 cell lines apoptosis Bcl-2 |
| 基金项目:CSCO-先声抗肿瘤血管靶向治疗科研基金（Y-S2014-016） |
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| 中文摘要: |
| 摘 要：［目的］ 观察热疗联合阿霉素对人多发性骨髓瘤细胞株ARH-77的体外增殖抑制、凋亡及Bcl-2表达的影响。［方法］ 采用MTT法确定阿霉素的工作浓度，以该浓度进行化疗或与热疗的联合，选择温度40℃、41℃及42℃，作用于ARH-77细胞。采用绘制肿瘤细胞生长曲线、MTT法检测对肿瘤细胞增殖的抑制作用；流式细胞仪检测肿瘤细胞凋亡及Bcl-2表达。［结果］ 阿霉素IC50为 8.16μg/ml，以此为实验的工作浓度。单纯热疗组60min对ARH-77细胞有抑制作用（P<0.01），并随温度增高而增强。单纯阿霉素化疗组对ARH-77细胞也有抑制作用。各热化疗组对ARH-77均有明显的抑制作用（P<0.01）。热疗组、化疗组及热化疗组的细胞凋亡率均较对照组显著升高，各组之间差异有统计学意义（P<0.01）；Bcl-2蛋白表达下降，各组之间也有显著性差异（P<0.01）。［结论］ 热疗联合阿霉素能增强对ARH-77细胞的体外抑制作用，提高肿瘤细胞的凋亡率，下调Bcl-2蛋白的表达，为热化疗的临床应用提供实验室依据。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the influence of hyperthermia combined with adriamycin on the inhibition of proliferation，apoptosis and Bcl-2 expression in multiple myeloma cell line ARH-77.［Methods］ The working concentration of adriamycin against ARH-77 was determined by MTT assay. The inhibition effect was evaluated by the growth curve of tumor cells and MTT assay. The apoptosis rate and Bcl-2 expression in ARH-77 were determined by flow cytometric analysis.［Results］ The IC50 of adriamycin in the experiment was 8.16μg/ml，which was defined as its working concentration. Each hyperthermia group for 60min showed obvious inhibition to ARH-77(P<0.01)，which increased with the increasing of temperature. The adriamycin chemotherapy group inhibited the growth of ARH-77 too (P<0.01). Each thermochemotherapy group showed obvious inhibition effect to ARH-77(P<0.01).Compared with control group，cell apoptosis rates increased and Bcl-2 expression decreased significanty in hyperthermia group，chemotherapy group and thermochemotherapy group，with significant difference among the groups(P<0.01).［Conclusion］ Hyperthermia combined with adriamycin chemotherapy might promote the inhibition effect to ARH-77，increase the apoptosis rate and down-regulate Bcl-2 expression，which provide laboratory evidence for the clinical application of thermochemotherapy. |
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