| 石 铖,张一昕,李 猛.去甲斑蝥素诱导人胃癌SGC-7901细胞凋亡的机制研究[J].中国肿瘤,2016,25(12):999-1003. |
| 去甲斑蝥素诱导人胃癌SGC-7901细胞凋亡的机制研究 |
| Norcantharidin Induces Apoptosis in Human Gastric Cancer Cells SGC-7901 |
| 投稿时间：2016-03-23 |
| DOI：10.11735/j.issn.1004-0242.2016.12.A011 |
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| 中文关键词: 去甲斑蝥素 胃癌 细胞周期 细胞形态 |
| 英文关键词:norcantharidin gastric cancer cell cycle cell morphology |
| 基金项目:河北中医学院青年科研基金（QNZ2015010） |
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| 中文摘要: |
| 摘 要：［目的］ 探讨去甲斑蝥素对人胃癌SGC-7901细胞抑制和诱导凋亡作用。［方法］ 采用不同浓度的去甲斑蝥素（noncantharidin，NCTD）作用于胃癌SGC-7901细胞，在扫描电镜下观察其形态特点，采用流式细胞术测定经NCTD作用后的细胞生长周期及凋亡率，Western-blot检测Bcl-2、Mcl-1、Bax的表达。［结果］ 扫描电镜下可见，SGC-7901细胞出现凋亡形态学改变。经去甲斑蝥素处理SGC-7901细胞后，实验组G2/M细胞明显多于对照组（P<0.05）。10μg/ml和20μg/ml浓度去甲斑蝥素均能诱导胃癌SGC-7901细胞凋亡(P<0.05)，其抑制率与NCTD浓度及作用时间相关。Western-blot检测Bcl-2、Mcl-1蛋白表达减少，Bax蛋白表达增加，呈明显的剂量关系。［结论］ 去甲斑蝥素对人胃癌SGC-7901细胞有抑制作用，可能与其阻滞细胞周期诱导细胞凋亡有关。 |
| 英文摘要: |
| Abstract：［Purpose］ To explore inhibition and apoptosis of norcantharidin on human gastric cancer SGC-7901 cell.［Methods］ Gastric cancer SGC-7901 cells were treated with different concentrations（5，10，20，40μg/ml） of norcantharidin(NCTD) for 24，48，72h in vitro， morphological characteristics of apoptosis were observed with elelectron microscope，the cell growth cycle and apoptosis rate were determined by flow cytometry. Bcl-2，Mcl-1 and Bax expression were detected with Western blot. ［Results］ Under the electron microscope，SGC-7901 cells appeared morphological changes of apoptosis. After SGC-7901 treated with norcantharidin，G2/M cells in the experimental group were significantly higher than that in control group(P<0.05). The 10μg/ml and 20μg/ml norcantharidin induced apoptosis of gastric cancer SGC-7901 cells(P<0.05)， in a concentration and time-dependeit manner. The expression of Bcl-2 and Mcl-1 protein expression was decreased，the Bax protein expression increased in a dose-dependent manner. ［Conclusion］ Norcantharidin has an inhibitory effect on human gastric cancer SGC-7901 cells，which is related to cell cycle arrest and apoptosis. |
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