| 林 琳,周刘祥,王晓春.MiRNA-424对人结肠癌细胞系Lovo细胞增殖及Rspo3基因表达的影响[J].中国肿瘤,2018,27(3):223-228. |
| MiRNA-424对人结肠癌细胞系Lovo细胞增殖及Rspo3基因表达的影响 |
| Effects of MicoRNA-424 on Proliferation and Rspo3 Gene Expression in Human Colon Cancer Lovo Cell Line |
| 投稿时间：2017-11-16 |
| DOI：10.11735/j.issn.1004-0242.2018.03.A012 |
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| 中文关键词: miR-424 结肠癌 Lovo细胞 增殖 Rspo3基因 |
| 英文关键词:miR-424 colon cancer Lovo cells proliferation Rspo3 gene |
| 基金项目:湖南省自然科学基金（10JJ5010）；中南大学研究生自主探索创新项目（2017zzts846） |
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| 中文摘要: |
| 摘 要：［目的］ 探讨miR-424对人结肠癌Lovo细胞增殖及Rspo3基因表达的影响。［方法］ 运用实时荧光定量PCR检测Lovo细胞和正常结肠上皮组织NCM460细胞中miR-424与Rspo3 mRNA 的表达水平。miR-424抑制剂（inhibitor）转染Lovo细胞后，观察细胞增殖能力，及miR-424、Rspo3 mRNA、miR-15/16/103表达水平，Western blot检测Rspo3蛋白表达水平。通过在线软件预测靶向Rspo3基因的miRNA，并用双荧光素酶报告基因实验验证。［结果］ MiR-424与Rspo3 mRNA在结肠癌Lovo细胞中过表达（P<0.05）。miR-424 inhibitor组Lovo细胞增殖受到明显抑制（P<0.05），且Rspo3基因表达量显著下降（P<0.05），miR-15/16/103均上调，其中miR-103上调最显著（P<0.05）。双荧光素酶报告基因实验验证miR-103可靶向调控Rspo3基因的表达。［结论］ miR-424抑制剂可阻滞Lovo细胞增殖且通过反馈性上调miR-103靶向抑制Rspo3基因的表达。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the effect of miR-424 on proliferation and RSPO3 gene expression in human colon cancer Lovo cell line. ［Methods］ Real-time quantitative PCR was used to detect the expressions of miR-424 and Rspo3 mRNA in colon cancer Lovo cells and normal colorectal epithelial NCM460 cells. After transfected with miR-424 inhibitor，negative control and liposome respectively，the proliferation of Lovo cells was detected by CCK-8 method. The expressions of Rspo3 mRNA and miR-15/16/103 were detected by real-time qRCR. The miRNAs targeting Rspo3 gene were predicted by prediction software and verified by dual luciferase reporter assay. ［Results］ MiR-424 and Rspo3 mRNA were overexpressed in Lovo cells (P<0.05). The proliferation of Lovo cells transfected with miR-424 inhibitor was significantly inhibited(P<0.05)，and the expression of Rspo3 gene was significantly decreased(P<0.05)，while miR-15/16/103 was upregulated(P<0.05). Dual luciferase reporter gene experiments confirmed that miR-103 targeted Rspo3 gene expression. ［Conclusion］ Inhibition of miR-424 can reduce the proliferation of Lovo cells and down-regulate the Rspo3 expression through the up-regulation of miR-103. |
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