| 房祥杰,张 彬,张德重.迷迭香酸通过MAPK/ERK信号通路对结肠癌细胞增殖及凋亡的影响[J].中国肿瘤,2018,27(4):306-310. |
| 迷迭香酸通过MAPK/ERK信号通路对结肠癌细胞增殖及凋亡的影响 |
| Effects of Rosmarinic Acid on Proliferation and Apoptosis of Colon Cancer Cells via MAPK/ERK Signaling Pathway |
| 投稿时间：2017-08-11 |
| DOI：10.11735/j.issn.1004-0242.2018.04.A011 |
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| 中文关键词: 迷迭香酸 结肠肿瘤 细胞增殖 细胞凋亡 MAPK/ERK信号通路 |
| 英文关键词:rosmarinic acid colon cancer cell proliferation apoptosis MAPK/ERK signaling pathway |
| 基金项目:河南省二○一四年科技发展计划（142102310047） |
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| 中文摘要: |
| 摘 要：［目的］ 探讨迷迭香酸通过丝裂原激活的蛋白激酶/胞外信号调节的蛋白激酶（MAPK/ERK）信号通路对结肠癌细胞增殖凋亡的影响。［方法］ 取对数生长期LS174T细胞，加入迷迭香酸（0、25、50和100μmol/L）处理24、48 和72h后，CCK-8法检测迷迭香酸对细胞增殖活性的影响。以100μmol/L迷迭香酸处理细胞，24、48 和72h后，采用流式细胞仪检测迷迭香酸对细胞周期和细胞凋亡率的影响；采用蛋白质印迹法检测细胞中Bax、Bcl-2和Cleaved Caspase-3蛋白的表达及ERK1/2磷酸化水平。［结果］ 迷迭香酸对LS174T细胞呈时间浓度依赖性抑制细胞增殖；100μmol/L迷迭香酸处理LS174T细胞不同时间后，与对照组相比，迷迭香酸组细胞中S期和G2/M期细胞所占比例明显降低，G0/G1期比例显著升高，但组间时间效应不显著（P>0.05）；而Bcl-2蛋白表达、ERK1/2磷酸化水平明显降低，细胞凋亡率、Bax和Cleaved Caspase-3蛋白表达量显著增强，均表现出明显的时效关系（P<0.05）。［结论］ 迷迭香酸能够抑制结肠癌LS174T细胞增殖，诱导细胞凋亡，其作用机制可能与抑制MAPK/ERK信号通路有关。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the effect of rosmarinic acid on the proliferation and apoptosis of colon cancer cells and its relation to MAPK/ERK signaling pathway. ［Methods］ The cultured human colorectal cancer LS174T cells in logarithmic growth phase were treated with rosmarinic acid（0，25，50 and 100μmol/L）for 24，48 and 72h，respectively. CCK-8 assay was used to detect the effect of rosmarinic acid on cell proliferation；flow cytometry was used to detect the effects of rosmarinic acid on cell cycle and cell apoptosis rate after LS174T cells were treated with 100μmol/L rosmarinic acid for 24，48 and 72h；the expressions of Bax，Bcl-2 and cleaved Caspase-3 proteins and phosphorylation level of ERK1/2 were detected by immunoblotting. ［Results］ Rosmarinic acid inhibited the proliferation of LS174T cells in a time and dose-dependent manner. Compared with the control group，the proportion of S phase and G2/M phase cells in rosmarinic acid treatment group decreased and that of G0/G1 phase increased significantly，but the time effect between the groups was not significant（P>0.05）. However，the expression of Bcl-2 protein and the level of ERK1/2 phosphorylation were significantly decreased，and the apoptosis rate，the expression of Bax and cleaved Caspase-3 protein were significantly increased in a time-dependent manner（P<0.05）. ［Conclusion］ Rosmarinic acid can inhibit the proliferation of human colorectal cancer LS174T cells and induce apoptosis，which may be related to the inhibition of MAPK/ERK signaling pathway. |
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