| 郭 苹,陈小兵,别良玉.siRNA沉默NOB1的表达对胃癌细胞凋亡及顺铂化疗敏感性的影响[J].中国肿瘤,2018,27(7):536-540. |
| siRNA沉默NOB1的表达对胃癌细胞凋亡及顺铂化疗敏感性的影响 |
| Effect of siRNA Silencing NOB1 Expression on Apoptosis and Chemosensitivity to Cisplatin in Gastric Cancer Cells |
| 投稿时间：2017-09-29 |
| DOI：10.11735/j.issn.1004-0242.2018.07.A010 |
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| 中文关键词: NOB1 胃癌 顺铂 凋亡 |
| 英文关键词:NOB1 gastric cancer cisplatin apoptosis |
| 基金项目:国家自然科学基金面上项目(81472714)；河南省医学科技重点攻关项目 |
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| 中文摘要: |
| 摘 要：［目的］ 探讨siRNA沉默酵母基因Noblp人类同源基因（NOB1）对胃癌细胞凋亡及顺铂化疗敏感性的影响。［方法］ 胃癌细胞MGC-803转染NOB1 siRNA和siRNA control为干扰组、对照组，同时以顺铂处理的两组细胞为顺铂＋干扰组、顺铂组，qRT-PCR和Western blot法检测NOB1表达，噻唑蓝法（MTT）检测细胞增殖，流式细胞仪检测细胞凋亡，Western blot检测信号转导与转录因子3(STAT3)、磷酸化STAT3（p-STAT3），比色法检测含半胱氨酸的天冬氨酸蛋白水解酶3（Caspase-3）活性。［结果］ 干扰组、顺铂组、顺铂＋干扰组细胞中NOB1 mRNA和蛋白水平下降，细胞存活率降低，细胞凋亡率升高，细胞中Caspase-3活性升高，p-STAT3/STAT3水平降低，与对照组相比差异具有统计学意义（P<0.05）。顺铂＋干扰组细胞凋亡率和Caspase-3活性明显高于干扰组、顺铂组，细胞存活率和p-STAT3/STAT3水平明显低于干扰组、顺铂组，差异均具有统计学意义（P<0.05）。［结论］ siRNA沉默NOB1基因促进胃癌细胞凋亡，增加顺铂敏感性，作用机制与p-STAT3/STAT3有关。 |
| 英文摘要: |
| Abstract：［Purpose］To investigate the effect of siRNA silencing NOB1 gene on apoptosis and chemosensitivity to cisplatin in gastric cancer cells. ［Methods］ Gastric cancer MGC-803 cells were transfected with NOB1 siRNA or siRNA control，then treated with or without cisplatin；thus the cells were divided into 4 groups：interference，control，interference+cisplatin and cisplatin groups. The expression of NOB1 was detected by RT-PCR and Western blot；cell proliferation was detected by MTT，apoptosis was detected by flow cytometry，the expression of STAT3 and p-STAT3 was detected by Western blot，and Caspase-3 activity was detected by colorimetric assay. ［Results］ The levels of NOB1 mRNA and protein decreased，cell viability decreased，the rate of apoptosis was increased，Caspase-3 activity was elevated，levels of p-STAT3/STAT3 were decreased in interference，interference + cisplatin and cisplatin groups，compared with the control group(all P<0.05). The apoptosis rate and Caspase-3 activity of interference + cisplatin group were increased，and the cell viability and p-STAT3/STAT3 levels were decreased more markedly than those in interference group and cisplatin group(P<0.05). ［Conclusion］ Silencing NOB1 gene by siRNA promotes apoptosis of gastric cancer cells and increases their sensitivity to cisplatin，which may be related to p-STAT3/STAT3 pathway. |
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