| 程 艳,崔金全,李 远.MicroRNA-506靶向Caveolin-1抑制绒毛膜癌细胞增殖和侵袭的研究[J].中国肿瘤,2019,28(3):227-233. |
| MicroRNA-506靶向Caveolin-1抑制绒毛膜癌细胞增殖和侵袭的研究 |
| MicroRNA-506 Inhibits the Proliferation and Invasion of Choriocarcinoma Cells via Targeting Caveolin-1 |
| 投稿时间：2018-07-26 |
| DOI：10.11735/j.issn.1004-0242.2019.03.A012 |
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| 中文关键词: 绒毛膜癌 microRNA-506 小窝蛋白1（caveolin-1） |
| 英文关键词:choriocarcinoma microRNA-506 caveolin-1 |
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| 中文摘要: |
| 摘 要：［目的］ 探讨microRNA-506 (miR-506)在绒毛膜癌中对绒毛膜癌细胞的作用及其作用机制。［方法］ 通过qRT-PCR检测绒毛膜癌和正常早孕绒毛临床标本中miR-506及caveolin-1表达量及相关性；将绒毛膜癌细胞JEG3和JAR按照miR-506 mimic（mimic）、miR-506 inhibitor（Inhi）、miR-506 mimic negative control（NC）和空白对照组（Blank）分别进行转染，通过MTT实验、Transwell实验和流式细胞术检测miR-506对绒毛膜细胞作用；通过双荧光素酶报告实验、qRT-PCR和Western blot检测miR-506、caveolin-1及相关蛋白的表达变化。［结果］ 与正常早孕绒毛相比，miR-506在绒毛膜癌组中表达显著降低（P<0.05），并与caveolin-1表达呈负相关。MiR-506 过表达明显抑制绒毛膜癌细胞的增殖、侵袭并促进凋亡。双荧光素酶报告实验表明miR-506过表达显著抑制野生型荧光素酶活性，qRT-PCR和Western blot结果显示miR-506过表达显著抑制caveolin-1表达，进而抑制相关蛋白表达。［结论］ MiR-506可能通过靶向caveolin-1抑制绒毛膜癌细胞的增殖和侵袭。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the effect of microRNA-506 (miR-506) on proliferation and invasion ability of choriocarcinoma and its mechanisms. ［Methods］ The expression of miR-506 and caveolin-1(CAV-1) in choriocarcinoma and normal early pregnancy villi was measured by qRT-PCR. Human choriocarcinoma JEG3 JAR cells were transfected with miR-506 mimic，miR-506 inhibitor or miR-506 mimic negative control (NC)，respectively. The cell proliferation，invasive ability and apoptosis were detected by MTT，transwell，and flow cytometry，respectively. The interaction，variation of miR-506 and CAV-1，the expressions of related proteins were detected by dual luciferase report assay，qRT-PCR and Western blot methods. ［Results］ Compared with normal early pregnancy villi，expression of miR-506 in choriocarcinoma significantly decreased(P<0.01) and it was negatively correlated with the expression of CAV-1. Dual luciferase report assay showed that miR-506 significantly inhibited activity of wild type luciferase in JEG3 and JAR cells. Overexpression of miR-506 significantly inhibited proliferation，invasion of choriocarcinoma cells and induced the cell apoptosis，and also significantly inhibited expression of CAV-1 and related proteins. ［Conclusion］ MiR-506 may inhibit proliferation and invasion of choriocarcinoma cells via targeting CAV-1. |
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