| 韦珏伶,向 骁,赵凌云.HSP90AA1/HSPA8在合并抑郁情绪的肝癌患者中的表达及临床意义[J].中国肿瘤,2019,28(5):387-394. |
| HSP90AA1/HSPA8在合并抑郁情绪的肝癌患者中的表达及临床意义 |
| Expression of HSP90AA1/HSPA8 in Hepatocellular Carcinoma Patients with Depression and Its Clinical Significance |
| 投稿时间：2018-11-20 |
| DOI：10.11735/j.issn.1004-0242.2019.05.A012 |
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| 中文关键词: 热休克蛋白 抑郁 肝细胞癌 血管内皮生长因子 |
| 英文关键词:heat shock protein depression hepatocellular carcinoma vascular endothelial growth factor |
| 基金项目:国家科技重大专项课题(2012ZX10002010001009)；广西卫计委中医药科技专项课题(GZLC16?鄄36)；广西医疗卫生适宜技术开发与推广应用课题(S201417?鄄03，S2018014)；广西科技重点研发计划项目（桂科AB18126042） |
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| 中文摘要: |
| 摘 要：［目的］ 探讨热休克蛋白(heat shock protein，HSP) 90AA1/HSPA8在合并抑郁情绪的肝癌患者中的表达及临床意义。［方法］ 收集2015年9月至2016年6月在广西医科大学附属肿瘤医院行肝切除术的肝细胞癌患者131例。术前采用抑郁自评量表（SDS）和医院焦虑/抑郁量表(HADS)评估患者的抑郁情况，根据是否存在抑郁分为无抑郁组（N=45）、轻度抑郁组(N=38)、中重度抑郁组(N=48)。通过实时荧光定量PCR、Western blot及免疫组织化学染色检测HSP90AA1/HSPA8及VEGF/VEGFR2-PI3K-AKT通路指标的表达情况，比较3组肝癌患者无病生存率并分析这些指标与预后的关系。［结果］ 中重度抑郁组肝癌患者HSP90AA1/HSPA8的表达显著高于无抑郁组和轻度抑郁组（P＜0.05）；中重度抑郁组无病生存率明显低于无抑郁组和轻度抑郁组（P＜0.05），轻度抑郁组和无抑郁组无病生存率差异无统计学意义。单因素和多因素分析结果均显示，血管侵犯和中重度抑郁是影响肝癌患者预后的独立危险因素（P＜0.05）。相对于无抑郁和轻度抑郁组，中重度抑郁组HSP90AA1、HSPA8、VEGF、VEGFR2、PI3K、AKT1的蛋白表达增加，Caspase9和BAD蛋白表达降低。［结论］ 中重度抑郁组肝癌患者HSP90AA1/HSPA8呈高表达，预后较差，可能与VEGF/VEGFR2-PI3K-AKT通路的激活有关。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the expression of HSP90AA1/HSPA8 in hepatocellular carcinoma patients with depression and its clinical significance. ［Methods］ The clinical data of 131 patients with hepatocellular carcinoma undergoing initial liver resection at the Affiliated Cancer Hospital of Guangxi Medical University from September 2015 to June 2016 were analyzed. Patients were assessed with self-rating depression scale（SDS）and hospital anxiety and depression scale(HADS) before surgery，and patients were divided into no depression group(N=45)，mild depression group(N=38) and moderate-to-severe depression group(N=48). The expressions of HSP90AA1/HSPA8 and VEGF/VEGFR2-PI3K-AKT pathway related factors(VEGF，VEGFR2，PI3K and AKT1) were detected by real-time RT-PCR，Western blot and immunohistochemistry. The disease-free survival(DFS) was compared among the three groups， and the relation of above factors with prognosis was analyzed. ［Results］ The mRNA and protein expressions of HSP90AA1/HSPA8 were significantly higher in the moderate-to-severe depression group than those in the no depression group and mild depression group（P<0.05），while rate of DFS was significantly lower in the moderate-to-severe depression group than that in the no depression and mild depression group（P<0.05）. Univariate analysis and multivariable analysis both identified vascular invasion and moderate-to-severe depression as independent prognostic factors for hepatocellular carcinoma patients(P<0.05）. Compared to no depression and mild depression groups，expressions of HSP90AA1，HSPA8，VEGF，VEGFR2，PI3K and AKT1 increased in the moderate-to-severe depression group，while the expressions of Caspase9 and BAD decreased. ［Conclusion］ Hepatocellular carcinoma patients with moderate-to-severe depression have poorer prognosis，in those patients the HSP90AA1/HSPA8 are expressed at high level，which may be activated by the VEGF/VEGFR2-PI3K-AKT pathway. |
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