| 武 玉,王益民,赵 敏.MiR-885对结直肠癌细胞奥沙利铂化疗敏感性的影响[J].中国肿瘤,2020,29(10):799-804. |
| MiR-885对结直肠癌细胞奥沙利铂化疗敏感性的影响 |
| Effect of MiR-885 on Sensitivity to Oxaliplatin in Colo-rectal Cancer Cells |
| 中文关键词 修订日期：2020-04-28 |
| DOI：10.11735/j.issn.1004-0242.2020.10.A013 |
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| 中文关键词: miR-885 结直肠癌 耐药 奥沙利铂 |
| 英文关键词:miR-885 colorectal cancer drug-resistance oxaliplatin |
| 基金项目: |
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| 中文摘要: |
| 摘 要：［目的］ 探究miR-885对结直肠癌细胞奥沙利铂化疗敏感性的影响。［方法］利用qRT-PCR 验证在耐药和敏感结直肠癌蜡块组织中miR-885的相对表达量；运用质粒转染方法沉默结直肠癌细胞系HCT116中miR-885基因，按照miR-885表达水平分为2组：miR-NC和miR-885 inhibitor组，并且利用qRT-PCR验证转染效率。将细胞分成4组，分别是阴性对照组(NC)、阴性对照加奥沙利铂组（L-OHP）、miR-NC加奥沙利铂组（L-OHP/miR-NC）和miR-885 inhibitor加奥沙利铂组（L-OHP/miR-885 inhibitor）。利用CCK-8法检测HCT116细胞活性；Transwell检测HCT116细胞的侵袭能力；流式细胞术检测HCT116细胞的细胞周期。［结果］ 相比于癌旁正常组织（1.31±0.36），耐药组织（2.75±0.82）中miR-885的表达量增加，而敏感组织（0.31±0.14）中miR-885的表达量降低。在miR-885组中miR-885表达水平（0.02±0.01）相对于miR-NC组（0.80±0.19）呈显著降低(P<0.05）。与L-OHP/miR-NC组（0.77±0.14）相比，L-OHP/miR-885 inhibitor组（0.48±0.23）细胞活性降低（P<0.05）。与L-OHP/miR-NC组（169±25）相比，L-OHP/miR-885 inhibitor组（28±9）细胞的侵袭数量减少（P<0.05）。与NC组G0/G1期（42.27±3.33）相比，L-OHP组（3.33±0.41）细胞比例降低（P<0.001），与NC组S期（32.19±2.29）相比，L-OHP组（53.29±3.04）细胞比例增多(P<0.05)，与NC组（16.32±2.29）G2/M期相比，L-OHP组（4.83±0.76）细胞比例减少(P<0.05)。同时，与L-OHP/miR-NC组（5.07±0.31）G0/G1期相比，L-OHP/miR-885 inhibitor组（45.59±1.95）细胞比例增加（P<0.001），与L-OHP/miR-NC组（42.71±2.58）S期相比，L-OHP/miR-885 inhibitor组（12.72±1.60）细胞比例降低（P<0.001），与L-OHP/miR-NC组(18.19±1.30)G2/M期相比，L-OHP/miR-885 inhibitor组（2.55±0.51）细胞比例减少（P<0.01）。［结论］ 沉默miR-885能够增强结直肠癌细胞HCT116对奥沙利铂化疗敏感性，miR-885可能成为结直肠癌治疗的新靶点。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the effect of miR-885 on chemotherapy sensitivity of colorectal cancer cells. ［Methods］ The relative expression of miR-885 was detected with qRT-PCR in drug sensitive and resistant colorectal cancer tissues. The human colon cancer HCT116 cells were transfected with miR-885 inhibitor(miR-885 inhibitor) or blank plasmid(miR-NC). Then the cells were divided into four groups：negative control group(NC)，negative control group plus oxaliplatin(L-OHP)，miR-NC plus oxaliplatin group(L-OHP/miR-NC) and miR-885 inhibitor plus oxaliplatin group(L-OHP/miR-885). CCK-8 method was used to detect the viability of HCT116 cells；Tran-swell method was used to detect the cell invasiveness；flow cytometry was used to detect the cell cycle. ［Results］ The expression of miR-885 in drug-resistant tissues(2.75±0.82) was higher than that in sensitive tissues(0.31±0.14). The expression level of miR-885 in miR-885 group(0.02±0.01) was significantly lower than that in miR-NC group(0.80±0.19)(P＜0.05). The results of CCK-8 showed that compared with L-OHP / miR-NC group(0.77±0.14)，the cell viability of L-OHP / miR-885 inhibitor group(0.48±0.23) decreased(P＜0.05). Compared with L-OHP / miR-NC group(169±25)，the number of migrant cells in L-OHP / miR-885 inhibitor group(28±9) decreased(P＜0.05). Compared with NC group(42.27±3.33) in G0/G1 phase，the cell proportion of L-OHP group(3.33±0.41) decreased(P＜0.001)，compared with NC group(32.19±2.29) in S phase，the cell proportion of L-OHP group(53.29±3.04) increased(P<0.05)，compared with NC group(16.32±2.29) G2/M phase，the cell proportion of L-OHP group(4.83±0.76) decreased(P<0.05). Compared with L-OHP/miR-NC group(5.07±0.31) in G0/G1 phase，the cell proportion of L-OHP/miR-885 inhibitor group(45.59±1.95) increased(P<0.001)，compared with L-OHP / miR-NC group(42.71±2.58) in S phase，the cell proportion of L-OHP/miR-885 inhibitor group(12.72±1.60) decreased(P<0.001)，compared with the G2/M phase of L-OHP/miR-NC group(18.19±1.30)，the cell proportion of L-OHP/miR-885 inhibitor group(2.55±0.51) decreased(P<0.01). ［Conclusion］ MiR-885 silencing can enhance the chemosensitivity of colon cancer HCT116 cells to oxaliplatin，indicating that miR-885 might be a novel target for colorectal cancer treatment. |
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