| 王晓坤,杨 欢,范金虎,等.基于林县营养干预试验人群开展的食管鳞癌代谢组学标志物探索[J].中国肿瘤,2023,32(2):154-160. |
| 基于林县营养干预试验人群开展的食管鳞癌代谢组学标志物探索 |
| Exploration of Biomarkers of Esophageal Squamous Cell Carcinoma Based on Metabolomics |
| 投稿时间：2022-10-28 |
| DOI：10.11735/j.issn.1004-0242.2023.02.A011 |
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| 中文关键词: 食管鳞癌 代谢组学 生物标志物 差异分析 |
| 英文关键词:esophageal squamous cell carcinoma metabolomics biomarkers differential analysis |
| 基金项目: |
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| 中文摘要: |
| 摘 要：［目的］ 开展巢式病例对照研究，通过代谢组学的手段分析得到差异代谢物，探索食管鳞癌发病前可能的代谢标志物。［方法］ 本研究基于1985年开展的林县营养干预试验随访队列，选择2001年及之后确诊为食管鳞癌者30例，并根据年龄（±3岁）、性别匹配至今仍存活的健康对照30名。1999年秋季至2000年春季的随访调查中获得了上述研究对象的血浆标本，使用LC-MS的方法进行代谢组学检测，对获得的代谢组特征进行分析。［结果］ 食管鳞癌组和对照组的基线人口学信息差异无统计学意义，但饮食偏好存在一定差异。代谢组检测数据稳定，代谢分析发现两组在代谢物分布上有明显差异，确定了包括谷氨酸、胆碱、溶血卵磷脂类、鸟氨酸在内的9种重要差异代谢物。［结论］ 食管鳞癌患者体内代谢物表达存在明显改变，某些含量波动的物质或可以成为食管鳞癌识别和诊断的潜在生物标志。 |
| 英文摘要: |
| Abstract： ［Purpose］ To explore biomarkers of esophageal squamous cell carcinoma (ESCC) using metabolomic approach. ［Methods］ A nested case-control study was conducted from autumn 1999 to spring 2000. The cohort of Linxian Nutrition Intervention Trial conducted starting in 1985 was used in the study， 30 patients who developed ESCC after 2001 and age of 30 years old (±3 years) and gender-matched healthy controls were enrolled from the cohort. The plasma specimens were obtained from all study subjects， and metabolomic assays were performed using the LC-MS method. ［Results］ There was no significant difference in baseline demographic information between ESCC patients and controls， but there were differences in dietary preferences between two groups. There were significant differences in metabolite distribution between the two groups， with nine differential metabolites， including glutamate， choline， lysophosphatidylcholine and ornithine. ［Conclusion］ Metabolite composition is significantly modified in patients with esophageal squamous cell carcinoma， and certain substances with fluctuating levels may be potential biomarkers for the identification and diagnosis of esophageal squamous carcinoma. |
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