| 李 鹏,吕晓东,常玉喜.PD-L1单抗对EGFR敏感性突变肺癌细胞PD-L1表达及对增殖的影响[J].肿瘤学杂志,2019,25(3):209-212. |
| PD-L1单抗对EGFR敏感性突变肺癌细胞PD-L1表达及对增殖的影响 |
| Effect of Anti PD-L1 Monoclonal Antibody on PD-L1 Expression and Proliferation in EGFR Sensitive Mutant Lung Cancer Cells |
| 投稿时间：2018-07-25 |
| DOI：10.11735/j.issn.1671-170X.2019.03.B007 |
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| 中文关键词: 肺肿瘤 皮生长因子受体 程序性死亡配体 PD-L1单抗 |
| 英文关键词:lung neoplasms skin growth factor receptor programmed death ligand PD-L1 monoclonal antibody |
| 基金项目:河南省医学科技攻关计划项目（2018020507） |
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| 中文摘要: |
| 摘 要：［目的］ 探讨PD-L1单抗对EGFR敏感性突变肺癌细胞PD-L1的表达及对增殖的影响。［方法］ 选取2017年5月至2018年8月在我院呼吸内科治疗的非小细胞肺癌患者60例为研究对象，同时选取同期来我院检查的健康人群30例为对照。采集外周静脉血，测定血清标本中游离PD-L1（sPD-L1）蛋白含量。建立肺癌细胞与T细胞共培养体系。［结果］ 肺鳞癌组与肺腺癌组sPD-L1的表达水平明显高于对照组（P<0.001），而肺鳞癌组与肺腺癌组sPD-L1表达水平无统计学差异（t=2.584，P=0.086）；野生组与突变组sPD-L1表达水平亦无统计学差异（t=2.215，P=0.097）。sPD-L1表达量与患者性别、年龄、分期无相关性（P＞0.05），而与患者的吸烟史、肿瘤转移相关（P＜0.05）。 EGFR敏感型sPD-L1表达率明显高于EGFR野生型（t=12.365，P<0.001）。加入PD-L1单抗后，EGFR的sPD-L1的表达水平有明显下降趋势，而EGFR野生型与治疗前无统计学差异（t=2.154，P=0.958）。PD-L1单抗使用后，PC9、HCC827等EGFR敏感型细胞株共同体系中T细胞明显增加。［结论］ sPD-1表达与EGFR敏感性无明显相关性，而与患者是否吸烟、肿瘤转移相关。与此同时，EGFR敏感突变肺癌细胞行PD-L1单抗干预后，sPD-L1的表达量明显降低，T细胞增殖明显。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effect of PD-L1 monoclonal antibody on the expression of PD-L1 and proliferation of EGFR-sensitive mutant lung cancer cells. ［Methods］ Sixty patients with non-small cell lung cancer(NSCLC) treated in our respiratory department from May 2017 to August 2018 were enrolled in the study，and 30 healthy subjects served as the control group. Peripheral venous blood was collected to determine the content of free PD-L1 in serum samples (sPD-L1). A co-culture system of lung cancer cells and T cells was established. ［Results］ The expression of sPD-L1 in patients with lung squamous cell carcinoma and those with lung adenocarcinoma was significantly higher than that in control group(P<0.001)，but there was no significant difference in the expression of sPD-L1 between lung squamous cell carcinoma group and lung adenocarcinoma group(t=2.584，P=0.086)，and there was no significant difference between wild tumor group and mutant tumor group (t=2.215，P=0.097). The expression of sPD-L1 was not correlated with gender，age and stage(P>0.05)，but correlated with smoking history and tumor metastasis(P<0.05). The expression rate of sPD-L1 in EGFR-sensitive type was significantly higher than that in EGFR-wild type(t=12.365，P<0.001) before the treatment with PD-L1 monoclonal antibody. After adding PD-L1 monoclonal antibody，the expression of mPD-L1 in EGFR-sensitive type decreased significantly，but there was no significant difference before and after treatment in wild-type EGFR type(t=2.154，P=0.958). After PD-L1 monoclonal antibody treatment，the number of T cells in co-culture system with EGFR-sensitive cell lines (C9，HCC827) was increased significantly. ［Conclusion］ The expression of sPD-1 is not correlated with EGFR sensitivity，but with smoking and tumor metastasis. Expression of sPD-L1 is decreased in EGFR-sensitive mutant lung cancer cells treated with PD-L1 monoclonal antibody，which is associated with proliferation of T cells. |
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