| 乔旭旭,毕明宏,张浩然.Avasimibe对Lewis肺癌小鼠抗肿瘤作用研究[J].肿瘤学杂志,2019,25(5):413-417. |
| Avasimibe对Lewis肺癌小鼠抗肿瘤作用研究 |
| Anti-tumor Effect of Avasimibe on Lewis Lung Cancer Mice |
| 投稿时间：2018-09-29 |
| DOI：10.11735/j.issn.1671-170X.2019.05.B007 |
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| 中文关键词: avasimibe ACAT-1 Lewis肺癌 抗肿瘤 |
| 英文关键词:avasimibe ACAT-1 Lewis lung carcinoma anti-tumor |
| 基金项目:安徽省卫生厅医学科研项目（09C171）；安徽省高校自然科学研究项目（KJ2016A483）；蚌埠医学院研究生创新课题（Byycxz1722）。 |
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| 中文摘要: |
| 摘 要：［目的］ 观察Avasimibe对Lewis肺癌小鼠模型肿瘤生长及转移的抑制作用,探索其可能的作用机制。［方法］ 选择24只C57BL/6小鼠，左前肢腋下接种Lewis肺癌细胞（LLC）构建Lewis肺癌小鼠模型，随机分成对照组、环磷酰胺（CTX）组、Avasimibe组、CTX+Avasimibe四组，每组6只。于接种肿瘤后第10d分别向Lewis肺癌小鼠模型腹腔注射生理盐水、CTX溶液、Avasimibe、CTX+Avasimibe溶液。至造模后35d，观察各组小鼠生存状态及移植瘤生长情况，称量小鼠体重、瘤重、胸腺重，计算抑瘤率、胸腺指数，观察肝肺转移情况，Western blot检测瘤组织ACAT-1的表达。［结果］ 与对照组比较，小鼠未出现体重减轻，各组瘤重均降低，CTX+Avasimibe组瘤重低于CTX组及Avasimibe组。 四组抑瘤率分别为0、53.45%、51.51% 和62.22%，与对照组比较，其余三组抑瘤率升高；与CTX组及Avasimibe组比较，CTX+Avasimibe组抑瘤率升高；与对照组比较，CTX组胸腺重显著性降低，Avasimibe组胸腺重显著性升高，CTX组胸腺指数显著性降低，与CTX组相比，Avasimibe组及CTX+Avasimibe组的胸腺重及胸腺指数均显著性增加。 对照组小鼠出现肝肺转移，CTX组、Avasimibe组及CTX+Avasimibe组未见肝肺转移情况；与对照组和CTX组比较，Avasimibe组及CTX+Avasimibe组ACAT-1蛋白表达降低。［结论］ Avasimibe在Lewis肺癌小鼠体内可有效发挥抑制肿瘤生长及转移的作用，而且还可以提高小鼠的免疫功能，这为肺癌治疗提供一个新靶点。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effect of Avasimibe on Lewis lung carcinoma in mice. ［Methods］ Lewis lung carcinoma cells were subcutaneously inoculated in 24 C57BL/6 mice，then the mice were randomly divided into 4 groups with 6 animals in each group：control group，cyclophosphamide(CTX) group，avasimibe group and CTX+ avasimibe group. On d10 after inoculation，the normal saline，CTX solution，avasimibe solution，or CTX plus avasimibe solution were intraperitoneally injected in 4 groups，respectively. On d35 after inoculation，the survival status of the mice，the growth of the transplanted tumor and the liver and lung metastasis were observed，the tumor weight，thymus weight were measured，the tumor inhibition rate，thymus index were calculated，and the expression of ACAT-1 in tumor tissue was detected.［Results］ Compared with the control group，the tumor weight of 3 treatment groups was significantly lower，that of the CTX+avasimibe group was the lowest. The tumor inhibition rates of the control group，CTX group，avasimibe group and CTX+ avasimibe group were 0，53.45%，51.51%，and 62.22%，respectively. The tumor inhibition rate of CTX+ avasimibe group was significantly lower than that of CTX group and the avasimibe group(P<0.05). Compared with the control group，the thymus weight and thymus index of CTX group were significantly decreased，while that of avasimibe group were significantly increased. Compared with the CTX group，the thymus weight and thymus index in the avasimibe group and the CTX+ avasimibe group were significantly increased. The metastatic lesions in the liver and lung were observed in the control group，while there was no liver and lung metastasis in the three treatment groups. Compared with the control group and the CTX group，the expression of ACAT-1 protein in avasimibe group and CTX+ avasimibe was significantly decreased. ［Conclusion］ Avasimibe can effectively inhibit tumor growth and metastasis，and improve the immune function in mice with Lewis lung carcinoma. |
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