| 孙满强,胡凯文,陈 琪.冷冻消融术对Lewis肺癌小鼠肿瘤微环境炎症因子的影响[J].肿瘤学杂志,2019,25(9):774-778. |
| 冷冻消融术对Lewis肺癌小鼠肿瘤微环境炎症因子的影响 |
| Effects of Cryoablation on Tumor Microenvironmental Inflammatory Factors in Lewis Lung Cancer Mice |
| 投稿时间：2019-02-24 |
| DOI：10.11735/j.issn.1671-170X.2019.09.B002 |
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| 中文关键词: 冷冻消融术 0℃复温 40℃复温 炎症因子 |
| 英文关键词:cryoablation 0℃ rewarming temperature 40℃ rewarming temperature inflammatory cytokines |
| 基金项目:国家自然科学基金（81403250）；北京中医药大学基本科研业务（2019-JYB-XS） |
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| 中文摘要: |
| 摘 要：［目的］ 探究不同复温温度下冷冻消融术对Lewis肺癌小鼠肿瘤微环境中炎症因子的影响。［方法］ 45只C57BL小鼠随机分为3组，每组15只，分别是对照组、T0组（复温至0℃）、T40组（复温至40℃）。实验组小鼠采用单循环冷冻—复温消融手术，当冰球覆盖至肿瘤边缘时，停止降温，分别复温至0℃和40℃。冷冻消融术后第14d对小鼠进行处死、取材，称量小鼠的体重和肿瘤重量，采用免疫组化的方法测量肿瘤组织中白细胞介素4(IL-4）、转化生长因子-β（TGF-β）、干扰素-?酌（IFN-?酌）和肿瘤坏死因子（TNF-?琢）的表达。［结果］ 各组小鼠体重在冷冻手术干预前并无显著性差异，术后14d对照组体重为（20.43±1.74）g，T0组体重为（18.59±0.98）g，T40组体重为（18.91±0.86）g。T0组和T40组体重均小于对照组（F=9.199，P<0.05）。术后第14天对照组瘤重为（1.83±2.59）g，T0组瘤重为（1.37±1.69）g，T40组瘤重为（1.54±1.00）g，T0组和T40组瘤重均低于对照组（F=0.23，P<0.05）。免疫组化结果以平均光密度值（IOD/Area）来表示，对照组IL-4表达量为0.102±0.015，T0组表达量为0.094±0.012，T40组表达量为0.101±0.013，T0组<对照组（P<0.05），T40组与对照组之间无统计学差异。对照组TGF-β表达量为0.129±0.036，T0组表达量为0.090±0.013，T40组表达量为0.110±0.039，T0组<对照组（P<0.05），T40组与对照组之间无统计学差异。对照组IFN-γ表达量为0.100±0.016，T0组表达量为0.121±0.036，T40组表达量为0.112±0.043，T0组>对照组（P<0.05），T40组与对照组之间无统计学差异。对照组TNF-α表达量为0.110±0.018，T0组表达量为0.129±0.033，T40组表达量为0.117±0.018，T0组>对照组（P<0.05），T40组与对照组之间无统计学差异。［结论］ 冷冻消融术对于残存瘤侵袭转移有抑制作用，其机制可能与调控肿瘤微环境中的炎症因子有关，相较于40℃复温，0℃在这一机制方面具有更好的调控效果。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effect of cryoablation at different rewarming temperatures on inflammatory factors in tumor microenvironment of Lewis lung cancer mice. ［Methods］ Forty-five C57BL mice were randomly divided into 3 groups，15 in each group，control group，T0 group(thawing to 0℃)，the T40 group(thawing to 40℃). The mice in the experimental group were treated with single cycle cryopreservation and rewarming ablation. When the puck reaches the edge of the tumor，stop the cooling. After wen to 0℃ and 40℃ respectively. Mice were sacrificed and sampled 14 days after cryoablation. The mice were weighed and the tumor was weighed. Testing the expression of IL-4、TGF-β、IFN-γ、TNF-α with immunohistochemistry. ［Results］ There was no significant difference in body weight between the groups before cryosurgery intervention.14 days after surgery，the weight of the control group was (20.43±1.74)g，the T0 group was (18.59±0.98) g，and the T40 group was(18.91±0.86)g. Both T0 group and T40 group had significantly lower body weight than that in control group(F=9.199，P<0.05). On the 14th day after surgery，the tumor weight of the control group was(1.83±2.59) g，that of the T0 group was (1.37±1.69)g，and that of the T40 group was(1.54±1.00)g，both of which were lower than those of the control group(F=0.23，P<0.05). Immunohistochemical results were represented by mean optical density(IOD/Area). The expression level of IL-4 in the control group was 0.102±0.015，that in the T0 group was 0.094±0.012，that in the T40 group was 0.101±0.013. The expression of T0that of control group（F=1.89，P<0.05）. There was no significant difference between T40 group and control group. The expression level of TNF-α in the control group was 0.110±0.018，that in the T0 group was 0.129±0.033，that in the T40 group was 0.117±0.018，The expression of T0>that of control group（F=6.041，P<0.05）. There was no significant difference between T40 group and control group. ［Conclusion］ Cryoablation can inhibit the invasion and metastasis of residual tumor. The mechanism may be related to the regulation of inflammatory factors in tumor microenvironment. Compared with 40℃ temperature，0℃ in this mechanism has better control effect. |
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