| 陈 静,董善武,陈咏丽,等.miR-640通过抑制SLIT1介导Wnt/β-catenin通路促进脑胶质瘤对替莫唑胺的耐药[J].肿瘤学杂志,2022,28(7):568-573. |
| miR-640通过抑制SLIT1介导Wnt/β-catenin通路促进脑胶质瘤对替莫唑胺的耐药 |
| miR-640 Promote Proliferation， Invasion and Resistance to Temozolomide in Glioma by Inhibiting SLIT1-mediated Wnt/β-catenin Pathway |
| 投稿时间：2021-12-29 |
| DOI：10.11735/j.issn.1671-170X.2022.07.B007 |
|
 |
| 中文关键词: 脑胶质瘤 替莫唑胺 miR-640 Wnt/β-catenin通路 |
| 英文关键词:glioma temozolomide miR-640 Wnt/β-catenin pathway |
| 基金项目:武汉市卫健委科研项目（WX21C05） |
|
| 摘要点击次数: 398 |
| 全文下载次数: 120 |
| 中文摘要: |
| 摘 要：［目的］评价miR-640介导Wnt/β-catenin通路对脑胶质瘤耐药细胞增殖、侵袭及替莫唑胺(temazolamide，TMZ)敏感性的影响。［方法］ 利用实时荧光定量聚合酶链反应（RT-qPCR）测定脑胶质瘤细胞内miR-640的表达。U87/TMZ细胞分为NC inhibitor组、 miR-640 inhibitor组和miR-640 inhibitor+si-SLIT1组。用不同浓度（15、30、60、120、240 、480和960 μmol/L）TMZ处理细胞，检测细胞对TMZ的耐药性；运用细胞计数试剂盒8（CCK-8）、Transwell实验检测U87/TMZ细胞增殖活力和侵袭力；用双荧光素酶报告基因实验验证miR-640与SLIT1的靶向关系。采用Western blot检测Wnt/β-Catenin信号通路相关蛋白表达水平。［结果］ miR-640在脑胶质瘤细胞中高表达，转染后miR-640 inhibitor组的miR-640表达水平、侵袭细胞数目（57±23 vs 153±31）、IC50值（131.61±8.97 vs 293.33±11.28）、增殖率（18.48±4.23 vs 43.84±8.94）均比NC inhibitor组低（P<0.05）。miR-640靶向调控SLIT1的表达，miR-640 inhibitor+si-SLIT1组β-catenin、c-myc、Cyclin D1蛋白表达水平均高于miR-640 inhibitor组（P<0.05）。［结论］miR-640通过SLIT1介导Wnt/β-catenin通路促进脑胶质瘤增殖、侵袭及对替莫唑胺的耐药性。 |
| 英文摘要: |
| Abstract： ［Objective］ To investigate the effects of miR-640-mediated Wnt/β-catenin pathway on proliferation， invasion and sensitivity to temazolamide(TMZ) in glioma cells. ［Methods］ The expression of miR-640 in glioma cells as determined by real-time fluorescence quantitative polymerase chain reaction(RT-qPCR). TMZ-resistant human glioma U87/TMZ cells were transfected with NC inhibitor， miR-640 inhibitor and miR-640 inhibitor+ si-SLIT1， respectively. U87/TMZ cells were treated with TMZ(15， 30， 60， 120， 240， 480 and 960 μmol/L) . Cell counting Kit 8(CCK-8) and Transwell assay were used to detect the proliferation and invasion of U87/TMZ cells. Dual luciferase reporter gene assay was used to verify the targeting relationship between miR-640 and SLIT1. Western blot was used to detect the expression levels of Wnt/β-catenin signaling pathway related proteins. ［Results］ miR-640 was highly expressed in glioma cells. The miR-640 expression level， number of invaded cells(57±23 vs 153±31)， IC50 value(131.61±8.97 vs 293.33±11.28) and proliferation rate(18.48±4.23 vs 43.84±8.94) in miR-640 inhibitor group were significantly lower than those in NC inhibitor group(P＜0.05). The miR-640 specifically regulated the expression of SLIT1， and the protein expression levels of β-catenin， c-myc and Cyclin D1 in miR-640 inhibitor+ si-SLIT1 group were higher than those in miR-640 inhibitor group(P＜0.05). ［Conclusion］ miR-640 may promote glioma proliferation， invasion and resistance to TMZ through SLIT1-mediated Wnt/β-catenin pathway. |
|
在线阅读
查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|